RNA
Part:BBa_K3081114
Designed by: Yiwen Wang Group: iGEM19_Peking (2019-10-16)
a 20bp sequence targeting the IHF(integration host factor) box on E.coli oriC
The 20 bp sgRNA targets to the integration host factor (IHF) binding site on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see BBa_K3081058
Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]
Reference:
[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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